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Functional analysis of the Leu658Pro mutation within the TLR2/TIR domain. The TLR2 mutation Leu658Pro was inserted into a plasmid for human TLR2. Human embryonic kidney 293 cells were then transfected with mutated as well as wild-type TLR2. Reporter genes RSV-β-galactosidase and Elam-NF-κB-luciferase were cotransfected. Cells were stimulated with synthetic bacterial lipoprotein analogue. As negative control, wild-type TLR2 was tested with medium alone.
