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. 2005 Dec 27;103(1):39–44. doi: 10.1073/pnas.0507740102

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Fig. 4. — Effect of GE81112 on 30S initiation complex formation. (A and B) Comparison of the inhibition caused by the indicated concentrations of GE81112 on translational activity (▪) and on 30S initiation complex formation (▴) (A) and by the indicated concentrations of the following known or presumed P-site inhibitors (B): GE81112 (▪), viomycin (□), edeine (▿), kasugamycin (▵), and pactamycin (). (C) Effect of the order of addition of GE81112 with respect to the translation components. The indicated concentrations of GE81112 were preincubated (5 min at 37°C) with 30S ribosomal subunits before the addition of all other components of the translational machinery (▾) or added after 70S initiation complex formation and before the ingredients required for translation elongation (•). Additional experimental details are given in Materials and Methods.

Inline graphic — Effect of GE81112 on 30S initiation complex formation. (A and B) Comparison of the inhibition caused by the indicated concentrations of GE81112 on translational activity (▪) and on 30S initiation complex formation (▴) (A) and by the indicated concentrations of the following known or presumed P-site inhibitors (B): GE81112 (▪), viomycin (□), edeine (▿), kasugamycin (▵), and pactamycin (). (C) Effect of the order of addition of GE81112 with respect to the translation components. The indicated concentrations of GE81112 were preincubated (5 min at 37°C) with 30S ribosomal subunits before the addition of all other components of the translational machinery (▾) or added after 70S initiation complex formation and before the ingredients required for translation elongation (•). Additional experimental details are given in Materials and Methods.