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. 2005 Dec 27;103(1):39–44. doi: 10.1073/pnas.0507740102

Fig. 5.

Fig. 5.

Inhibition of 30S initiation complex formation by GE81112. (A) Kinetics of fMet-tRNA binding to 30S ribosomal subunits programmed with 022mRNA in the absence (▾) and in the presence of 0.25 μg/ml (0.37 μM) (•), 0.5 μg/ml (0.75 μM) (▴), and 1 μg/ml (1.5 μM) (▵) GE81112. (B) Codon dependence and initiation factor dependence of GE81112 inhibition of fMet-tRNA binding to 30S ribosomal subunits as a function of Mg2+ concentration. The experiment was performed in the presence of the indicated concentration of MgCl2 as described in Materials and Methods in a complete system (♦) or in systems in which IF1, IF2, and IF3 (▾), mRNA (▪), and IF3 (▴) were omitted.