Comparison of growth, hypusine synthesis, and hypusine/deoxyhypusine content in DOHH-null and parent strains. The S. cerevisiae DOHH-null strain and the parental strain, BY4743, were cultured at 28°C. (A) SDS/PAGE after labeling with [1,8-3H]spermidine·HCl. (B) Ion exchange chromatographic separation of labeled hypusine and deoxyhypusine from labeled eIF5A proteins. (C) Growth curves. (D) Fluorometric detection of hypusine and deoxyhypusine in total cell protein (29).