Fig. 5.

Pharmacodynamic monitoring of HIF prolyl hydroxylase inhibitor. (A) Bioluminescent images of ROSA26 ODD-Luc/+ mice 6 h after administration of vehicle, Compound A (100 mg/kg), or FG-4383 (100 mg/kg) by oral gavage. (B) Bioluminescent images of ROSA26 ODD-Luc/+ mice before and 6 h after administration of FG-4383 by oral gavage at indicated doses. Color bar indicates photons/(cm²·sec·steradian) with minimum and maximum threshold values. (C) Immunoblot analysis of tissue extracts prepared from wild-type (WT) and ROSA26 ODD-Luc/+ (ODD) mice 6 h after treatment with 100 mg/kg FG-4383.