Fig. 6.

Detection of antibodies in breast cancer patient serum. (A) Slide image obtained from fluorescence scan after cancer patient serum assay with IgM detection. The grids contain sugars 1–4a printed in the top row from left to right, 1–4b in the middle row, and 5 and 6 in the bottom row, similar to Fig. 5A (B) Binding specificities of antibodies in cancer patient serum to Globo H analogs 1–4a, 5, and 6. Relative fluorescence intensities observed in spots containing 100 μM of glycan is plotted for IgM (filled bars) and IgG (empty bars) binding.