Fig. 3.
Increased production of IgE and IgG2a in mice adoptively transferred with Btk-deficient dendritic cells. WT and btk–/– BMDCs were incubated with 100 μg/ml OVA or PBS for 3 h, and the BMDCs (3 × 105 cells) were adoptively transferred to B6 mice. Total IgE, IgG2a, and IgG2b levels in sera were measured 7 days later. Data represents the mean ± SEM and analyzed by two-way ANOVA. *, P < 0.05, and **, P < 0.005 (vs. the PBS control of the same genotype); †, P < 0.05 (vs. the WT control). Similar results are reproduced in three independent experiments.