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. 2006 Jan 3;34(1):e1. doi: 10.1093/nar/gnj001

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Analysis of recombination time-course. Several C10 subclones were obtained and their recombination rates analyzed over a 7-week period. The recombination rate obtained for five of them (10.2, 10.3, 10.5, 10.6 and 10.7) was analyzed by several techniques. (A) Non-recombined (NR) and recombined (R) DNA fragments amplified by PCR. (B) Detection of β recombinase expression and histone H1, as control, by western blot. (C) Luciferase activity. Western blot and luciferase activity were measured in the fifth week.