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RT-PCR analysis of AnoACE gene expression. RNA was extracted from glucose fed adult females, blood fed adult females, eggs, larvae, pupae and adult males. Control templates for amplification were genomic DNA except for AnoACE2 primers when the template was DNA from cDNA clone 19600449622752 (MRA-467-39). Amplification products were observed for AnoACEs 2, 3, 7 and 9 only.
