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A, proteins of Actinobacillus seminis separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Lane 2, whole-cell extracts; lane 3, outer membrane proteins (OMPs); lane 4, cytoplasmic membrane proteins (CPs); lane 5, inner membrane proteins (IMPs). B, corresponding results of Western blot testing with rabbit antibody against A. seminis. In all the figures, lane 1 consists of molecular mass markers.
