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Concentration of controls in dilution 1 pools for modified latin square experiments
| Pool | Concentration Group A (Controls 1–24) | Concentration Group B (Clones 25–48) | Concentration Group C (Clones 49–72) | Concentration Group D (Clones 73–96) |
| Pool 7 | 125 | 1 | 5 | 25 |
| Pool 8 | 25 | 125 | 1 | 5 |
| Pool 9 | 5 | 25 | 125 | 1 |
| Pool 10 | 1 | 5 | 25 | 125 |
Concentration is given as mass ratios, so that "125" represents 1:125,000 or 1 ng of RNA transcript per 125 ng of background RNA where the spike amount is adjusted for its length.
