Table 6.

Concentration of controls in dilution pools for expanded range experiments

Pool	Conc. A Dil. 2	Conc. B Dil. 2	Conc. C Dil. 2	Conc. D Dil. 2	Conc. A Dil. 3	Conc. B Dil. 3	Conc. C Dil. 3	Conc. D Dil. 3	Conc. A Dil. 4	Conc. B Dil. 4	Conc. C Dil. 4	Conc. D Dil. 4
Pool 7	250	2	10	50	500	4	20	100	5,000	40	200	1,000
Pool 8	50	250	2	10	100	500	4	20	1,000	5,000	40	200
Pool 9	10	50	250	2	20	100	500	4	200	1,000	5,000	40
Pool 10	2	10	50	250	4	20	100	500	40	200	1,000	5,000

Concentration is given as mass ratios, so that "250" represents 1:250,000 or 1 ng of RNA transcript per 250 ng of background RNA, where the spike amount is adjusted for its length.

Concentrations of the initial stock, "Dilution 1" pools are shown in Table 4.