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. 2006 Jan 9;103(3):750–755. doi: 10.1073/pnas.0509690103

Fig. 5.

Fig. 5.

ChxR binds and activates transcription of chlamydial chxR. (A) ChxR binds DNA upstream of chlamydial genes in gel mobility-shift assays containing increasing concentrations of ChxR. Arrows indicate shifted complexes. (B) Transcriptional start site (+1) for chxR extended 23 bp upstream of the translation initiation codon (underlined). –10 and –35 promoter consensus sequences are boxed. (C) DNase I protection footprinting of ChxR at the chxR (Left) and infA (Right) promoters. (Left) Lanes 1 and 8 contain DNase I-only ladders. Lanes 2–6 show 2-fold increases of ChxR from 450 nM to 7.2 μM. Lane 7 contains 10.2 μM. (Right) Lanes 1 and 8 show DNase I-only ladders. Lanes 2–7 show 2-fold increases of ChxR from 225 nM to 7.2 μM. The numbers to the right of each panel represent the coordinates of ChxR protection from the transcription and translation start sites of chxR and infA, respectively.