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. 2006 Jan;140(1):91–101. doi: 10.1104/pp.105.071670

Figure 5.

Figure 5.

Transcriptional response of TaRAN1 to IAA treatment in wheat and effect of auxin on root development in transgenic Arabidopsis plants. A, RNA gel-blot analysis of 10−7 m/L IAA-responsive expression of TaRAN1 in wheat. The bars represent the relative amount of the normalized expression of TaRAN1 after IAA treatment for different times. B, Effect of IAA (10−10 or 10−7 m) on growth of primary roots in transgenic plants of Arabidopsis. The transgenic plants treated with IAA showed suppressed primary root growth as compared with wild-type plants. C, Effect of IAA (10−10 or 10−7 m) on lateral root numbers in transgenic Arabidopsis plants. The number of lateral roots of transgenic plants was increased compared with wild-type plants after IAA (10−10 or 10−7m) treatment. B and C, All plates were allowed to grow for 10 d to determine the effect of various concentrations of auxin on root length and lateral root production after vernalization. Results are presented as average values ± se from three experiments. More than 12 roots were used in each experiment. Asterisk (*), Significant difference, P < 0.01.