Figure 4.

RT-PCR of wild-type and atem6-1 RNA. Total RNA extracted from 10 seeds per sample was reverse transcribed using a tailed oligo(dT) primer and subsequently subjected to PCR using ATEM6 exon-specific primers. A portion of the RT-PCR reaction was run on a 1.2% agarose gel. Note this is not a quantitative measure of mRNA levels. Lane 1, RNA markers (sizes shown to the left); lane 2, wild type; lane 3, atem6-1 mutant; lane 4, ATEM6 genomic clone; and lanes 5 and 6, no RT controls for wild type and atem6-1 mutants, respectively.