Table II.

Kinetic constants of E. coli-expressed OsEPSPS and P106L mutant

Kinetic constants of wild-type EPSPS and P106L were obtained from the least-squares fitting that was carried out by using Igor Pro 4.00 software. Kinetic parameters are expressed as mean value ± sd derived from the best fit.

Kinetic Constants	OsEPSPS	OsEPSPS-P106L
Specific activity (U/mg)	10.032 ± 0.613	10.225 ± 0.599
IC50 (glyphosate; μm)	8.982 ± 0.799	104.606 ± 38.948
Km (PEP; μm)	19.940 ± 0.822	88.260 ± 9.480
Ki (glyphosate; μm)	0.057 ± 0.021	4.020 ± 0.087
Vmax (U/mg)	10.562 ± 0.490	10.663 ± 0.346
Ki/Km (PEP)	0.003	0.045