GUS staining patterns of transgenic Arabidopsis containing pPRF2-5′ and pPRF2e-p2i constructs at various incubation time points. Seedlings grown on Murashige and Skoog medium for 3 d were incubated for 1, 4, 8, and 12 h, respectively, and the reaction was stopped and the tissues cleared by incubation with 100% ethanol.