Figure 8.

Effect of the first intron on the expression of the Arabidopsis profilin gene family. A, Schematic representation of construction for transient expression. GP at the end of the construct name indicates a vector for transient expression. The white box is vegetative genes; the gray box is reproductive genes; the white or gray box with e is the first exon of each gene; the black box with GUS is GUS ORF; the thin lines with p1i, p2i, p3i, p4i, and p5i are the first introns of PRF1, PRF2, PRF3, PRF4 and PRF5, respectively; the white or gray box with gene name pr is the promoter region of each gene; ATG is the start codon. B, Relative normalized GUS activity in percentage. A plus (+) indicates the presence of an intron within the construct; a minus (−) indicates no intron. Each transfection was performed at least three times. GUS activity was normalized with cotransfected luciferase activity and relative activity of pPRF2eGP was set to 100%. Bars are means ± sd.