Figure 4.

Deduced VvCytoB5 (TC45693) amino acid sequence and comparison with the cytochrome b₅ protein DIF-F (Q9ZSP7) from petunia and other related sequences. A, Sequence comparison of the deduced amino acid sequence of TC45693 from grapevine and the cytochrome b₅ protein DIF-F (Q9ZSP7) from petunia. Identical amino acids are boxed in black, and similar amino acids are boxed in gray. B, Phylogenetic tree constructed with several cytochrome b₅-related sequences recovered by using the deduced amino acid sequence of TC45693 in a BLAST algorithm on the GenBank and EMBL databases. VvCytoB5 (TC45693) is shown in bold, and the tree was constructed as described in Figure 2. The scale bar represents 0.5 substitutions per site, and the numbers next to the nodes are bootstrap values from 100 replicates.