Table III.

Kinetic properties of Arabidopsis ll-DAP-AT

The reverse reaction contained in 1 mL 100 μmol HEPESKOH (pH 7.5), 0.3 μmol NADPH, 50 μmol NH₄Cl, 0.5 μmol ll-DAP, 5 μmol 2-OG, 16 μg CtDdh, and pure ll-DAP-AT. The reaction was incubated at 30°C and the decrease in A₃₄₀ was measured. The forward assay conditions were as described in the Figure 2 legend. V_max is in μmol min⁻¹ mg⁻¹ protein and K_cat is in s⁻¹. The kinetic constants were calculated by nonlinear regression analysis using GraphPad Prizm version 4.03.

Assay	Vmax	Kcat	Substrate	Km
Reverse	22.3 ± 0.3	17.6	ll-DAP	67 ± 2 μm
			2-OG	8.7 ± 0.3 mm
Forward	0.38 ± 0.01	0.3	THDPA	38 ± 4 μm
			Glu	1.9 ± 0.1 mm