Table I.
Substrate oxidation by pea seed and epicotyl mitochondria at low temperatures
Seed or epicotyl mitochondria respiration was measured with several substrates at low temperatures. Data correspond to an average of one to three repeats in a single experiment. State 3 oxidation rates are indicated in nanomoles O2 per minute per milligram protein, and the RCR is indicated in brackets. nd, Not detected.
| Temperature
|
State 3 Rate (RCR)
|
|||||
|---|---|---|---|---|---|---|
| NADH
|
Succinate
|
Malate-Glu
|
||||
| Seed | Epicotyl | Seed | Epicotyl | Seed | Epicotyl | |
| 5°C | 35.6 (3.2) | 105.6 (3.3) | 33.2 (3.5) | 69.3 (2.7) | 9.6 (2.4) | 29.6 (2.3) |
| 0°C | 18.8 (3.1) | 40.0 (2.1) | 8.4 (2.3) | 5.1 (1.0) | nd | nd |
| −3.5°C | 10.8 (3.3) | 22.5 (1.8) | 2.4 (1.0) | nd | – | – |