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. 2026 May 29;20:1799391. doi: 10.3389/fnins.2026.1799391

FIGURE 7.

Figure containing five panels labeled A to E. Panel A shows a western blot with bands for VEGFR-1, VEGFR-2, YAP1, Cleaved Caspase 3, and Actin, comparing control, Aβ, and VEGFR1-Si conditions. Panel B presents four bar graphs showing fold changes in VEGFR-1, VEGFR-2, YAP1, and Cleaved Caspase 3 expression across conditions, with significant differences indicated by asterisks. Panel C is a bar graph comparing percent survival at 24, 48, and 72 hours among four groups: control, VEGFR-1 Si, Aβ, and VEGFR-1 Si plus Aβ, with statistical significance marked. Panel D shows a bar graph of percent survival at 24, 48, and 72 hours for Lacz and EGLT groups, where EGLT survival decreases significantly over time. Panel E is a bar graph comparing mRNA expression of VEGFR-1 and Hif1α normalized to actin in control and Aβo-treated samples, both showing significant increases with Aβo.

VEGFR-1 knockdown prevents Ab 1-42 oligomer -induced neuronal death in SH-SY5Y cells. (A) Representative western blot image showing increased protein levels of VEGFR-1 and cleaved caspase3 and decreased VEGFR-2 and YAP1 levels in response to Ab1-42 oligomers (Ab, 5 μb, 5esentative west h in SH-SY5Y cells and siRNA mediated VEGFR-1 knockdown partially restored VEGFR-2 and YAP-1 levels. (B) Quantification of western blot bands from (A) showing fold change of the protein levels. (C) MTT assay showing decreased cell survival in the SH-SY5Y neurons in response to Ab1-42 oligomers (Ab, 5 μb, 5ssay showing decreased ce, 48, and 72 h and increased survival after siRNA mediated VEGFR-1 knockdown. (D) MTT assay showing increased neuronal toxicities/ cell death in the SH-SY5Y neurons in response to transfection with VEGFR-1 “EGLT” construct for 24, 48, and 72 h. (E) mRNA expression of VEGFR-1 and HIF1-a in SH-SY5Y cells after 24 h Ab1-42 oligomers (Abo, 5 μβ?, 5 ?λιγ?μερMean ± SD. *p<0.05, ** p < 0.01, ***p < 0.0001 (Student’s t-test). n = 3 independent experiments.