Table 2. Comparison between construct 1 and 2 of frequencies in targeting and expression of human βA-globin gene after repair.
Expression of hemoglobin A in targeted clones
|
||||
---|---|---|---|---|
Targeting construct | Targeting frequency | Crossover frequency at β6 | Yes | No |
Construct 1 | 8/74 | 7/8 | 6, 13, 34, 42, 51 | 5, 40 |
Construct 2 | 4/50 | 1/4 | 41 | 11, 43, 44 |