. 2006 Jan 3;103(2):466–471. doi: 10.1073/pnas.0502090103

Table 2. Stimulation of [³⁵S]GTPγS binding to hNPFF2R membranes by NPFF and ligands selected from the screening.

	hNPFF2
Ligands	EC₅₀, nM	Maximal activation, %
NPFF	22.5 ± 1	205 ± 5
RF2	>10,000	133 ± 7
RF9	>100,000	—
RF 48	1950 ± 450	145 ± 10.5
RF 49	694 ± 103	122 ± 6

Maximal activation is expressed as the percentage of basal [³⁵S]GTPγS binding, and values represent mean ± SEM from at least two separate experiments performed in triplicate. —, not determined.

Table 2. Stimulation of [35S]GTPγS binding to hNPFF2R membranes by NPFF and ligands selected from the screening.

Table 2. Stimulation of [³⁵S]GTPγS binding to hNPFF2R membranes by NPFF and ligands selected from the screening.