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. 2006 Jan 3;103(2):443–448. doi: 10.1073/pnas.0503839103

Fig. 2.

Fig. 2.

Transcriptional regulation of PPARγ and BACE1 by inflammatory cytokines and NSAIDs. (A) Modulation of BACE1 and PPARγ steady-state mRNA levels by IFN-γ (1 ng/ml) + TNF-α (30 ng/ml) is reversed with ibuprofen (IBU) (10 μM) in wild-type but not in knockout cells, shown by semiquantitative RT-PCR analysis of BACE mRNA. (B) Quantification of BACE1 steady-state mRNA levels in four experiments performed in PPARγ wild-type, heterozygous, and knockout cells. (C) Quantification of PPARγ steady-state mRNA levels in four experiments performed with the same samples. Columns represent mean ± SEM. (D) N2a-Sw cells transfected with PPARγ1 luciferase reporter construct were stimulated with IFN-γ (1 ng/ml) + TNF-α (30 ng/ml) with or without IBU (10 μM) or INDO(10 μM) (n = 3). *, P ≤ 0.05 ANOVA followed by a Tukey post hoc test.