Table 1. Characterization of a few representative Mtb H37Rv PA-824-resistant mutants.
| Minimal inhibitory concentration, μM
|
|||||||
|---|---|---|---|---|---|---|---|
| No. | Name | Phenotype | Genotype* | PA-824 | PA-647 | CGI-17341 | CGP-70520 |
| 1 | H37Rv | FGD1+; F420+; 824+ | – | 0.4–0.8 | 0.1–0.2 | 0.4 | 0.1 |
| 2 | H37Rv-T3 | FGD1-; F420+; 824- | na | >100 | >100 | >100 | >100 |
| 2a | H37Rv-T3-fgd1 | FGD1+; F420+; 824+ | fgd1::attP | 0.8 | 0.2 | 0.8 | 0.2 |
| 3 | H37Rv-5A1 | FGD1+; F420-; 824- | Δ1fbiC; Δ2PDIM | >100 | >100 | >100 | >100 |
| 3a | H37Rv-5A1-fbiC | FGD1+; F420+; 824+ | Δ1fbiC; Δ2PDIM; fbiC::attP | 0.4 | 0.2 | 0.8 | 0.2 |
| 4 | H37Rv-5A2 | FGD1+; F420- | fbiCV630E; fadD26D76A | >100 | >100 | >100 | >100 |
| 5 | H37Rv-7A2 | FGD1+; F420-; FO++; 824- | Unknown | >100 | >100 | >100 | >100 |
| 5a | H37Rv-7A2-fbiAB | FGD1+; F420+; 824+ | fbiAB::attP | 0.8 | 0.2 | 0.8 | 0.2 |
| 6 | H37Rv-T2 | FGD1+; F420+; 824- | Rv3547A76E | >100 | >100 | 0.8 | 3.2 |
| 6a | H37Rv-T2–3547 | FGD1+; F420+; 824+ | Rv3547A76E; Rv3547::attP | 1.6 | 0.2–0.4 | 0.4 | na |
| 7 | H37Rv-14A1 | FGD1+; F420+; 824- | Δ3Rv3547; Δ4ppsB | >100 | >100 | 0.8 | 3.2 |
| 7a | H37Rv-14A1–3547 | FGD1+; F420+; 824+ | Δ3Rv3547; Δ4ppsB; Rv3547::attP | 0.8–1.6 | 0.2 | 0.4 | na |
| 8 | H37Rv-Tn-824R#1 | FGD1+, F420+; 824- | Rv3547Y89ochre; 2258008::Tn | >100 | >100 | na | na |
| 9 | H37Rv-Tn-824R#4 | FGD1+, F420+; 824- | Rv3547::Tn | >100 | >100 | 0.8 | na |
| 9a | H37Rv-Tn-824R#4–3547 | FGD1+, F420+ | Rv3547::Tn; Rv3547::attP | 0.8 | 0.8 | 0.8 | na |
na, Not analyzed; 824+/- refers to PA-824 metabolism; Δ1, 400-bp deletion; Δ2, 66-kbp deletion; Δ3, a single bp deletion; Δ4, ≈1-kbp deletion.
*
Characterized by whole-genome CGS analysis, Tn-mutagenesis approach or complementation experiments