Fig. 4.

Subcellular localization of eYFP–NUP133 fusion protein in the nuclear rim. (A and B) Fluorescent image of a control eYFP localization in nup133-1.(C) Light microscopy of B with nucleus marked by arrow and highlighted in insert. (D, E, H, I, K, and L) Fluorescent images of eYFP–NUP133 fusion protein showing nuclear rim localization in nup133-1 mutant background. (E–K) Single cell localisation of eYFP–NUP133 in the nuclear rim. (E) Root hair cell showing nuclear rim localization of eYFP–NUP133. (F) Corresponding light microscopy with nucleus marked by arrow and highlighted in Inset. (H) Fluorescent overlay of G light microscopy with nuclei marked by arrows. Note the surface view of one nucleus showing punctate localization of eYFP–NUP133 fusion protein. (K) Overlay of corresponding fluorescent image of eYFP–NUP133 (I) and light microscopy image (J). Note the punctate eYFP–NUP133 localization. (L) Localization of eYFP–NUP133 in nuclear rims of cells in a transgenic root tip; note the cell files. (A and D) Epidermal cells. (B, C, E, and F) Root hair cells. (Scale bar: 50 μm.)