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Effect of yohimbine on guanine oxidation in the C24 → U24 mutant TfR IRE. (Left) Lanes 1–6, [RuO2+] = 100 μM. [yohimbine]: Lane 1, 0 μM; lane 2, 1 μM; lane 3, 5 μM; lane 4, 10 μM; lane 5, 20 μM; lane 6, 40 μM; lane 7, RNA-only control; lane 8, aniline-treated control. (Right) Quantitations of [32P]RNA bands. Cleavage intensity is in arbitrary units normalized to the intensity at G0.
