Fig. 4.

Dependence of QFRET signals on varied RNAs and varied target sites in intact HL-60 cells. Probe sequences are shown in Fig. 2. All probes had 2′-OMe RNA backbone. Cells were treated with QFRET probes (200 nM) and analyzed by FC under the same conditions as in Fig. 3. Relative FRET intensities were calculated from medians from 50,000 cells. The median with scrambled probes was used as a reference standard.