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. 2005 Dec 13;4:34. doi: 10.1186/1475-2859-4-34

Figure 2.

Figure 2

Comparison of the wt and I48A mutant EYFP soluble yields using the different pETM vectors. A) Small-scale affinity purification of two EYFPs (wild type and mutant I48A) expressed in BL21(DE3) bacterial cells and using the following vectors: pETM10, pETM14, pETM30, pETM11, pETM20, pETM22, pETM33, pETM44, pETM50, pETM52, pETM55, pETM66, pETM80, pETM54, pETM60, pETM70, pETM82. B) Small scale affinity purification of constructs expressed by pETM20 under different growth conditions and the alternative bacterial strain BL21(DE3) pLysS. C) Small-scale purification of wt EYFP expressed in pRSET and pETM10 vectors. D) Large-scale purification pattern of wt EYFP expressed in pETM11.