Table 1.
Sequences of the digoxigenin-conjugated sequence-specific oligonucleotide probes (SSOPs). The SSOP-ID named the sequences used in detection of the haemoglobin and G6PD single nucleotide polymorphisms (SNPs).
| SSOP ID | SSOP sequence1 | Nucleotide substitution | Amino acid substitution |
| HbB | |||
| HbA-6: | 5' TGACTCCTGAGGAGAAGT | ||
| HbS-6: | 5' TGACTCCTGTGGAGAAGT | A18T | E6V |
| HbC-6: | 5' TGACTCCTAAGGAGAAGT | G17A | E6K |
| HbA-26 | 5' TTGGTGGTGAGGCCCTGG | ||
| HbE-26: | 5' TTGGTGGTAAGGCCCTGG | G77A | E26K |
| G6PD | |||
| 68V | 5' CACCTTCATCGTGGGCTA | ||
| 68M | 5' CACCTTCATCATGGGCTA | G202A | V68M |
| 126N | 5' GCCACATGAATGCCCTCC | ||
| 126D | 5' GCCACATGGATGCCCTCC | A376G | N126D |
1: Letters in bold indicate the nucleotide-substitutions