Figure 2.

Effect of APOBEC3 proteins on IAP retrotransposition and cell viability. (A) HeLa cells were transfected with the IAP retrotransposition indicator plasmid pDJ33/440N1neo^TNF, together with an APOBEC3 expression plasmid or a control plasmid expressing β-arrestin. A derivative of pDJ33/440N1neo^TNF lacking a functional pol gene served as the negative control. Cells were subjected to selection in G418 and resistant colonies counted 17 days after transfection. This experiment is representative of the data compiled in Figure 1. (B) Same as (A) except that HeLa cells were co-transfected with pcDNA3, which encodes a neo cDNA, instead of with pDJ33/440N1neo^TNF. The negative in this case is a mock transfection.