Table 1.
Relative levels of C to T editing induced in retrotransposed IAP genome sequences by co-expressed APOBEC3 proteins
| hA3A (22 200) | A | T | C | G | hA3B (5800) | A | T | C | G | hA3G (4550) | A | T | C | G |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | – | 1 | 0 | 0 | A | – | 0 | 0 | 2 | A | – | 0 | 0 | 0 |
| T | 0 | – | 4 | 0 | T | 0 | – | 2 | 0 | T | 0 | – | 0 | 0 |
| C | 0 | 3 | – | 0 | C | 0 | 186 | – | 0 | C | 1 | 79 | – | 0 |
| G | 0 | 0 | 0 | – | G | 1 | 0 | 0 | – | G | 0 | 0 | 0 | – |
G418 resistant colonies obtained upon co-transfection of HeLa cells with the pDJ33/440N1neoTNF IAP indicator construct, and plasmids expressing hA3A, hA3B or hA3G, were harvested and used to prepare DNA. IAP genomic sequences were then PCR amplified, cloned and sequenced. The total number of bases sequenced is shown in parentheses, with mutations indicated in the grid. Predicted bases are shown on the left and the observed bases across the top.