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. 2006 Jan 10;34(1):120–129. doi: 10.1093/nar/gkj418

Figure 2.

Figure 2

LrpC promotes looped and transcomplexes on dsDNA. EcoRI-linearized pUC18 dsDNA [1.8 nM (A) or 0.3 nM (B)] was incubated with increasing concentrations of LrpC in the presence of 3 U of T4 DNA ligase for 3 h a room temperature. The deproteinized DNA was analyzed by agarose gel electrophoresis followed by ethidium bromide staining. LrpC concentrations are 20–80 nM in (A) (lanes 3–5 and 7–9); 80 and 160 nM in (B) (lanes 2 and 3) and 10–160 nM (lanes 5–9). Positions of Form II, Form III and multimers are indicated. In (A), the controls are shown (Form I and II in lane 1, and molecular marker in lane 10).