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. 2006 Jan 10;34(1):140–151. doi: 10.1093/nar/gkj430

Figure 2.

Figure 2

Time courses for killing of wild-type and repair-deficient S.cerevisiae by 5-FU. Yeast were shaken in 150 µM 5-FU for given times and then diluted and plated. (A) Single mutants lacking enzymes along the uracil BER pathway have distinct 5-FU survival curves. Ung1 and rad27 yeast are protected from the effects of 5-FU while apn1 yeast are more sensitive to the drug relative to wild type. (B) Apn1apn2ntg1ntg2 yeast is more sensitive to 5-FU relative to wild-type and the apn1 strain. (C) HR deficient rad51 yeast are modestly protected against 5-FU as compared with wild-type yeast. (D) The NER and mismatch repair-deficient rad2 and msh2 yeast show no increased sensitivity relative to wild type at this concentration of 5-FU.