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GC-MS quantification of U and 5-FU incorporation into the genomic DNA of yeast grown in the absence and presence of 5-FU. Cells were incubated in media containing 5-FU such that 70–90% cell killing was achieved. Genomic DNA was then isolated and digested with E.coli Ung to release U and 5-FU. (A) Uracil levels detected in the presence and absence of 5-FU. (B) 5-FU levels detected in the presence and absence of 5-FU.
