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. 2006 Jan 10;34(1):140–151. doi: 10.1093/nar/gkj430

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© The Author 2006. Published by Oxford University Press. All rights reserved

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5-FU incorporation into RNA. Wild-type yeast were grown in media in the absence and presence of 5-FU and cellular RNA was isolated and digested to its constituent nucleosides using mung bean nuclease and calf intestinal phosphatase. A small peak corresponding to 5-fluorouridine is visible in the HPLC spectrum of the digested RNA from the drug-treated cells (middle panel, inset). This peak is not seen with yeast grown in the absence of 5-FU (upper panel, inset). The lower panel shows the elution pattern for authentic nucleoside standards.