Figure 3.

Migration and chemotaxis of mouse embryonic fibroblasts expressing Endo180ΔEx2–6. (A) Wild-type (+/+) and ΔEx2–6/ΔEx2–6 mouse embryonic fibroblasts (MEFs) were analysed in migration assays, as described in the Methods section. The migratory paths, shown as trajectory plots, are from one, representative experiment where n was the number of cells in the experiment. The bar chart shows mean ± s.e.m. migratory speed for all cells analysed from three experiments. (B) Chemotactic response of +/+ and ΔEx2–6/ΔEx2–6 MEFs to a concentration gradient of urokinase-type plasminogen activator (uPA; 10 nM in the outer well of the Dunn chamber). The chemoattractant source is shown as a red asterisk. Migratory paths are shown as trajectory plots. The circular histograms show the proportion of cells with a migratory direction lying within each 18° interval. The red arrows represent the mean direction of migration, the green segments represent the 95% confidence interval determined by the Rayleigh test for unimodal clustering of direction. n, number of cells assayed.