T3 treatment increases histone H4 acetylation
specifically at the T3-response elements of T3-response
genes in pre-metamorphic tadpoles. Chromatin isolated from tail or intestine of
T3-treated stage NF55 tadpoles (treated with 10 nM T3 for
48 h) was immunoprecipitated (IP) with antibodies against acetylated histone H4
(AcH4) and analysed by PCR, as described for Fig. 1. Each
experiment was carried out at least twice. EF1a, elongation factor
1a; IFABP, intestinal fatty acid binding protein;
T3, thyroid hormone (triiodothyronine); TH/bZIP, a basic
leucine-zipper Th-response gene; TRb, thyroid hormone receptor
b.