Figure 3.
Effects of T3 on Rpd3 and NCoR co-repressor expression and recruitment on T3-response elements of T3-response genes in pre–metamorphic tadpoles. (A) Rpd3 and NCoR protein levels in tail nuclei are not affected by T3 treatment. Western blot analysis of protein extracts from the tail nuclei of tadpoles treated with 10 nM T3 for 48 h. (B) Chromatin isolated from tails of T3-treated tadpoles (treated with 10 nM T3 for 48 h) was immunoprecipitated (IP) with antibodies against Rpd3 or NCoR and analysed by PCR, as described for Fig. 1. Pre-immune serum (Pre-I) was used as a control for antibody specificity. The data represent one of several independent experiments with identical results. EF1a, elongation factor 1a; IFABP, intestinal fatty acid binding protein; NCoR, nuclear co-repressor; T3, thyroid hormone (triiodothyronine); TH/bZIP, a basic leucine-zipper Th-response gene; TRb, thyroid hormone receptor b.