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. 2003 Sep;4(9):872–876. doi: 10.1038/sj.embor.embor931

Figure 2.

Figure 2

The high level of occupancy of the PHO8 site by Gcn4 does not contribute to the regulation of PHO8 expression, or that of the upstream KRE2 gene. (A) The messenger RNA levels of the PHO8 gene from cells grown in either high- or low-phosphate media (HP and LP, respectively) are not affected by the presence of either low (WT/M) or high (WT/AT) levels of Gcn4, or by the absence (gcn4) of this protein. (B) mRNA levels of the KRE2 gene in wild-type (WT) and gcn4 strains grown in either minimal (M) or histidine starvation (AT) media. Actin mRNA (ACT1) was used as loading control in both experiments.