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. 2003 Sep 19;4(10):1000–1005. doi: 10.1038/sj.embor.embor942

Figure 2.

Figure 2

Binding kinetics of COPI proteins. Recovery, after bleaching the Golgi pool, of (A,B) ADP ribosylation factor 1 (ARF1) with and without aluminium fluoride, (C,D) ARF GTPase-activating protein 1 (ARFGAP1) with and without aluminium fluoride, and (E,F) ε-COP (ε-coat-protein) with and without aluminium fluoride (black symbols) with best fits (red lines). Whereas in untreated cells the typical recovery times T for ARF1 and ARFGAP1 were quite similar (A, TARF1 ≈ 10 s; C, TARFGAP1 ≈ 9 s), COPI showed a markedly slower recovery (E, TCOPI ≈ 20 s). Applying aluminium fluoride approximately doubled the recovery time for ARF1 (B, T ≈ 20 s), whereas ARFGAP1 showed only a 30% slower recovery (D, T ≈ 13 s), and coatomer was locked onto the membrane (F). WT, wild type.