Skip to main content
. 2003 Oct 1;4(12):1182–1189. doi: 10.1038/sj.embor.7400018

Figure 2.

Figure 2

VAMP4 binds exclusively to the μ1a subunit of AP-1 via its dileucine motif. (A) GST-VAMP4 or the LL/VV mutant were pretreated with or without CK2, bound to glutathione beads, incubated with BAMC and processed as in Fig. 1B. Mutations in the dileucine motif abolish AP-1 binding. (B) Yeast was co-transformed with VAMP42–60 and adaptins. A direct interaction of VAMP42–60 with the μ1a subunit of AP-1, but not with other adaptins, was observed. (C) Mutation of the dileucines in VAMP42–60 abolishes μ1a interaction.