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. 2003 Nov 21;4(12):1175–1181. doi: 10.1038/sj.embor.7400029

Figure 2.

Figure 2

Centrosome organization is normal in mbk-2 mutants but chromosome segregation is impaired. (A) Time-lapse GFP series from four-dimensional analyses of wild-type (WT) and mbk-2(dd5ts) embryos expressing histone and γ-tubulin::GFP. Time (s) is relative to nuclear envelope breakdown (NEB). Embryos are shown at corresponding stages to those in Fig. 1A. In the mutant, centrosome labelling (arrows) appears unchanged, but lagging chromosomes are visible from early anaphase to the two-cell stage (arrowheads). (B) Snapshots taken shortly after NEB from time-lapse recordings of WT and mbk-2(dd5ts) embryos expressing TAC-1::GFP. The mutant shows normal GFP expression. (C) Late-anaphase embryos stained for ZYG-9 (green) and DNA (blue). In the mutant, ZYG-9 expression is unaffected, but a failure in chromosome segregation is visible. (D) Distance between centrosomes (μm) in one-cell embryos versus time, observed in embryos expressing γ-tubulin::GFP as shown in (A). Four embryos per genotype were tracked. DIC, differential interference contrast.