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. Author manuscript; available in PMC: 2026 Jun 15.
Published in final edited form as: Nat Rev Drug Discov. 2025 Mar 18;24(7):543–569. doi: 10.1038/s41573-025-01158-9

Table 2 |.

Fibrosis monitoring in clinical trials: established or proposed end points

End point status Technology/measure used Comments Proposed use
Lung
Established FVC Provides a reproducible measure of disease severity and is the measure of choice for longitudinal assessment of disease progression Regulator-preferred end point of choice in phase III trials
Established Six-minute walk distance Frequently used in trials High variability; influenced by fitness, cardiovascular disease, pulmonary hypertension and mobility issues; often technically difficult for trial centres to perform
Proposed CT Image-based analysis of fibrosis progression Multiple imaging tools are in development, but none has been adequately validated
Proposed Blood biomarkers Several blood-based markers that track with disease progression and that change with treatment have been identified None has yet been fully validated
Gut
Proposed PRO Recommended for use in clinical trials Improvement in obstructive symptoms
Proposed Stricture radiology index Morphological evaluation of stricture Improvement in stricture morphology
Kidney
Established Histopathological quantification in kidney biopsy samples Recommended for use in clinical trials Assessment of fibrosis progression and/or reversal
Established eGFR slope Recommended for use in clinical trials as fibrosis correlates well with kidney function; however, large cohorts and long follow-ups might be needed Assessment of kidney function
Skin (SSc)
Established Modified Rodnan Skin Score Frequently used in clinical trials Assessment of dermal thickness over time and/or in response to therapy
Proposed Ultrasound-based quantification of dermal thickness Requires further validation Assessment of dermal thickness over time and/or in response to therapy
Liver
Established Histological assessment in liver biopsy samples Standardized histological scoring system Staging of fibrotic injury, progression or regression of fibrosis
Proposed (limited) Blood biomarkers; imaging biomarkers FDA workshop in 2023 provided guidance for early-stage clinical trials and for screening for high-risk patients before liver biopsy

CT, computed tomography; FVC, forced vital capacity; eGFR, estimated glomerular filtration rate; PRO, patient-reported outcome; SSc, systemic sclerosis.