Fig. 4.

Menin is important for methylation of H3K4 in the Hoxa9 locus and for binding of MLL and cdh1 to the locus. (A) A diagram of the murine Hoxa9 locus. (B and C) Myeloid cells harboring Men1^l/l;Cre-ER were treated with DMSO or 4-OHT, and then processed for ChIP assays by using the indicated antibodies. Results are representative of two independent experiments. (D) Men1 excision induced by 4-OHT was confirmed by Western blotting using an antimenin antibody. Equal loading of the samples was revealed by blotting for Lamin C.