FIG. 2. and 3.

Immunofluorescent imaging of microtubule disruption in drug-treated C. neoformans cells. Cells were fixed 0 (A), 0.5 (B), 1.5 (C), 2.5 (D), and 3.5 (E) h after treatment with 7.9 μM auristatin PHE (1.5 times the MIC) (Fig. 2) or 0.3 μM nocodazole (1.5 times the MIC) (Fig. 3). After 3.5 h, tubulin remnants accumulated in two discrete regions in the mother cell and the daughter cell (arrows). Bar, 5 μm.