FIG. 4.

Nuclear orientations of C. neoformans cells treated with auristatin PHE (A) or nocodazole (B). Cells were fixed 3.5 h after treatment with DMSO alone for controls or with 0.03 to 8 times the MIC of either drug (0.2 to 42.1 μM auristatin PHE; 0.006 to 1.6 μM nocodazole). Nuclei were either in the mother cell (M), in the daughter cell (D), in both (M/D), or in the neck (N), or two nuclei were present in the bud (D/D) or in the mother cell (M/M). Bars, means ± standard errors of the means for three independent experiments.