FIG. 5.

Standard fluorescent imaging of inhibition of nuclear migration and division in drug-treated C. neoformans cells. Chitin staining using wheat-germ agglutinin conjugated with TRITC revealed chitin rings at the bud neck (arrow) and bud scars (arrowhead) on previously divided mother cells (asterisk). The TRITC label was merged with DAPI labeling. Cells were fixed 3.5 h after treatment with either DMSO alone (control) (A), 7.9 μM auristatin PHE (1.5 times the MIC) (B), or 0.3 μM nocodazole (1.5 times the MIC) (C). Bar, 5 μm.