FIG. 2.

(A) Steady-state fluorescence polarization measurements (p value) in an S. cerevisiae WT strain and its erg mutants. Measurements were carried out with intact cells by using DPH as the fluorescent probe at excitation and emission wavelengths of 360 and 450 nm, respectively, as described in Materials and Methods. The values are means ± standard deviations (indicated by the bars) of three independent experiments. (B) Extracellular R6G concentration measured in S. cerevisiae WT and its erg mutants at 60-min interval. Deenergized yeast cells were incubated with R6G for 1 h, after which the cells were harvested and the extracellular concentration of R6G in the supernatant was determined spectrophotometrically by measuring the absorbance at 527 nm. The values are means ± standard deviations (indicated by the bars) of three independent experiments. (C) Accumulation of [³H]FLC in deenergized S. cerevisiae WT and its erg mutants at 60-min intervals. The values are means ± standard deviations (indicated by the bars) of three independent experiments.